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plenticrispr v2 control  (Addgene inc)


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    Structured Review

    Addgene inc plenticrispr v2 control
    Plenticrispr V2 Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenticrispr+v2/lentiCRISPR+v2+(Plasmid+%2352961)/pm42881259-203-18-21
    Average 96 stars, based on 6234 article reviews
    plenticrispr v2 control - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells.
    Article Snippet: HEK293T cells knocked out for Gq/11 or G12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTGAGCGGCTGATCCCTCAA), RDX (GTTTCACTTACCGCTGGGGT), MSN#1 (GAGACAAGTTGCTCCCGCAG), MSN#2 (AAGCTTACCTGAGCATGCCA), SLK (CAATTTGATATCTCCATCTA), and LOK (CATGATTGAGTTCTGTCCAG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described (Leguay et al, 2021).

    Article Title: CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.
    Article Snippet: .. Oligonucleotides were cloned into either the pX602-AAV-Cre sgRNA backbone for CRISPR–Cas9-induced acute gene knockout in mouse liver or the pLentiCRISPR V2 (Addgene, 52961) and pLentiGuide Blast for genome editing in cell lines, according to established protocols described previously48. .. The pX602-AAV-Cre sgRNA construct was derived from the pX602 vector (Addgene, 61593) in our previous study49.

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells
    Article Snippet: HEK293T cells knocked out for G q/11 or G 12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTG AGC GGC TGA TCC CTC AA), RDX (GTT TCA CTT ACC GCT GGG GT), MSN#1 (GAG ACA AGT TGC TCC CGC AG), MSN#2 (AAG CTT ACC TGA GCA TGC CA), SLK (CAA TTT GAT ATC TCC ATC TA), and LOK (CAT GAT TGA GTT CTG TCC AG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described ( ).

    CRISPR:

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells.
    Article Snippet: HEK293T cells knocked out for Gq/11 or G12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTGAGCGGCTGATCCCTCAA), RDX (GTTTCACTTACCGCTGGGGT), MSN#1 (GAGACAAGTTGCTCCCGCAG), MSN#2 (AAGCTTACCTGAGCATGCCA), SLK (CAATTTGATATCTCCATCTA), and LOK (CATGATTGAGTTCTGTCCAG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described (Leguay et al, 2021).

    Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction.
    Article Snippet: .. Genome-wide CRISPR KO screen for myddosome assembly regulators To express Cas9 in the parental MyD88–AGF iBMDMs, the sgRNA expression cassette was removed from pLentiCRISPR-v2 (52961, Addgene; a gift from F. Zhang)61 by digesting with KpnI-HF (R3142, NEB) and EcoRI-HF (R3101, NEB) and religating with annealed repair oligonucleotides that restore the restriction sites using Quick Ligation Kit (M2200, NEB). .. Next, the resulting plasmid was digested with BamHI-HF (R3136, NEB) and RsrII (R0501, NEB) to remove the PuroR gene and a bsr open reading frame (ORF) conferring blasticidin resistance amplified from pLenti-dCAS-VP64_Blast (61425, Addgene; a gift from F. Zhang)55 inserted using Gibson assembly to obtain pLenti-CRISPR-Blast-no-sgRNA.

    Article Title: CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.
    Article Snippet: .. Oligonucleotides were cloned into either the pX602-AAV-Cre sgRNA backbone for CRISPR–Cas9-induced acute gene knockout in mouse liver or the pLentiCRISPR V2 (Addgene, 52961) and pLentiGuide Blast for genome editing in cell lines, according to established protocols described previously48. .. The pX602-AAV-Cre sgRNA construct was derived from the pX602 vector (Addgene, 61593) in our previous study49.

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells
    Article Snippet: HEK293T cells knocked out for G q/11 or G 12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTG AGC GGC TGA TCC CTC AA), RDX (GTT TCA CTT ACC GCT GGG GT), MSN#1 (GAG ACA AGT TGC TCC CGC AG), MSN#2 (AAG CTT ACC TGA GCA TGC CA), SLK (CAA TTT GAT ATC TCC ATC TA), and LOK (CAT GAT TGA GTT CTG TCC AG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described ( ).

    Selection:

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells.
    Article Snippet: HEK293T cells knocked out for Gq/11 or G12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTGAGCGGCTGATCCCTCAA), RDX (GTTTCACTTACCGCTGGGGT), MSN#1 (GAGACAAGTTGCTCCCGCAG), MSN#2 (AAGCTTACCTGAGCATGCCA), SLK (CAATTTGATATCTCCATCTA), and LOK (CATGATTGAGTTCTGTCCAG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described (Leguay et al, 2021).

    Article Title: Loss of the kinases TBK1 and IKKε sensitizes target cancer cells to T cell killing through an IFN-γ–mediated inflammatory death program
    Article Snippet: .. Compound knockouts of B16 cells were generated by sequential lentiviral transduction with pLenticrispr v2 (a gift from Dr. Feng Zhang, Addgene #52961, puromycin selection), lenti-sgRNA blast (a gift from Dr. Brett Stringer, Addgene #104993, blasticidin selection), lenti-sgRNA neo (a gift from Dr. Brett Stringer, Addgene #104992, G418 selection), and lenti-sgRNA hygro (a gift from Dr. Brett Stringer, Addgene #104991, hygromycin selection). .. At each knockout and antibiotic selection stage, a corresponding non-targeting control guide was introduced using the same vector.

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells
    Article Snippet: HEK293T cells knocked out for G q/11 or G 12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTG AGC GGC TGA TCC CTC AA), RDX (GTT TCA CTT ACC GCT GGG GT), MSN#1 (GAG ACA AGT TGC TCC CGC AG), MSN#2 (AAG CTT ACC TGA GCA TGC CA), SLK (CAA TTT GAT ATC TCC ATC TA), and LOK (CAT GAT TGA GTT CTG TCC AG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described ( ).

    Sequencing:

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells.
    Article Snippet: HEK293T cells knocked out for Gq/11 or G12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTGAGCGGCTGATCCCTCAA), RDX (GTTTCACTTACCGCTGGGGT), MSN#1 (GAGACAAGTTGCTCCCGCAG), MSN#2 (AAGCTTACCTGAGCATGCCA), SLK (CAATTTGATATCTCCATCTA), and LOK (CATGATTGAGTTCTGTCCAG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described (Leguay et al, 2021).

    Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells
    Article Snippet: HEK293T cells knocked out for G q/11 or G 12/13 or every Gα subunit were obtained from Asuka Inoue (Tohoku University, Japan). .. Hs578T cell clones knocked out for ezrin, radixin, moesin, or SLK/LOK were obtained by CRISPR-Cas9, followed by clonal dilution after selection with 2 μg/ml puromycin (#540222; EMD Millipore) for 48 h. The following sequence guides were inserted in plentiCRISPR-v2 (#52961; Addgene) digested with BsmBI (#R0739; New England Biolabs): EZR (CTG AGC GGC TGA TCC CTC AA), RDX (GTT TCA CTT ACC GCT GGG GT), MSN#1 (GAG ACA AGT TGC TCC CGC AG), MSN#2 (AAG CTT ACC TGA GCA TGC CA), SLK (CAA TTT GAT ATC TCC ATC TA), and LOK (CAT GAT TGA GTT CTG TCC AG). .. For ebBRET experiments, HEK293T cells were transfected using linear polyethyleneimine (PEI, #43896; Alfa Aesar) as previously described ( ).

    Genome Wide:

    Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction.
    Article Snippet: .. Genome-wide CRISPR KO screen for myddosome assembly regulators To express Cas9 in the parental MyD88–AGF iBMDMs, the sgRNA expression cassette was removed from pLentiCRISPR-v2 (52961, Addgene; a gift from F. Zhang)61 by digesting with KpnI-HF (R3142, NEB) and EcoRI-HF (R3101, NEB) and religating with annealed repair oligonucleotides that restore the restriction sites using Quick Ligation Kit (M2200, NEB). .. Next, the resulting plasmid was digested with BamHI-HF (R3136, NEB) and RsrII (R0501, NEB) to remove the PuroR gene and a bsr open reading frame (ORF) conferring blasticidin resistance amplified from pLenti-dCAS-VP64_Blast (61425, Addgene; a gift from F. Zhang)55 inserted using Gibson assembly to obtain pLenti-CRISPR-Blast-no-sgRNA.

    Gene Knockout:

    Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction.
    Article Snippet: .. Genome-wide CRISPR KO screen for myddosome assembly regulators To express Cas9 in the parental MyD88–AGF iBMDMs, the sgRNA expression cassette was removed from pLentiCRISPR-v2 (52961, Addgene; a gift from F. Zhang)61 by digesting with KpnI-HF (R3142, NEB) and EcoRI-HF (R3101, NEB) and religating with annealed repair oligonucleotides that restore the restriction sites using Quick Ligation Kit (M2200, NEB). .. Next, the resulting plasmid was digested with BamHI-HF (R3136, NEB) and RsrII (R0501, NEB) to remove the PuroR gene and a bsr open reading frame (ORF) conferring blasticidin resistance amplified from pLenti-dCAS-VP64_Blast (61425, Addgene; a gift from F. Zhang)55 inserted using Gibson assembly to obtain pLenti-CRISPR-Blast-no-sgRNA.

    Article Title: CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.
    Article Snippet: .. Oligonucleotides were cloned into either the pX602-AAV-Cre sgRNA backbone for CRISPR–Cas9-induced acute gene knockout in mouse liver or the pLentiCRISPR V2 (Addgene, 52961) and pLentiGuide Blast for genome editing in cell lines, according to established protocols described previously48. .. The pX602-AAV-Cre sgRNA construct was derived from the pX602 vector (Addgene, 61593) in our previous study49.

    Expressing:

    Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction.
    Article Snippet: .. Genome-wide CRISPR KO screen for myddosome assembly regulators To express Cas9 in the parental MyD88–AGF iBMDMs, the sgRNA expression cassette was removed from pLentiCRISPR-v2 (52961, Addgene; a gift from F. Zhang)61 by digesting with KpnI-HF (R3142, NEB) and EcoRI-HF (R3101, NEB) and religating with annealed repair oligonucleotides that restore the restriction sites using Quick Ligation Kit (M2200, NEB). .. Next, the resulting plasmid was digested with BamHI-HF (R3136, NEB) and RsrII (R0501, NEB) to remove the PuroR gene and a bsr open reading frame (ORF) conferring blasticidin resistance amplified from pLenti-dCAS-VP64_Blast (61425, Addgene; a gift from F. Zhang)55 inserted using Gibson assembly to obtain pLenti-CRISPR-Blast-no-sgRNA.

    Ligation:

    Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction.
    Article Snippet: .. Genome-wide CRISPR KO screen for myddosome assembly regulators To express Cas9 in the parental MyD88–AGF iBMDMs, the sgRNA expression cassette was removed from pLentiCRISPR-v2 (52961, Addgene; a gift from F. Zhang)61 by digesting with KpnI-HF (R3142, NEB) and EcoRI-HF (R3101, NEB) and religating with annealed repair oligonucleotides that restore the restriction sites using Quick Ligation Kit (M2200, NEB). .. Next, the resulting plasmid was digested with BamHI-HF (R3136, NEB) and RsrII (R0501, NEB) to remove the PuroR gene and a bsr open reading frame (ORF) conferring blasticidin resistance amplified from pLenti-dCAS-VP64_Blast (61425, Addgene; a gift from F. Zhang)55 inserted using Gibson assembly to obtain pLenti-CRISPR-Blast-no-sgRNA.

    other:

    Article Title: Human MutLα activates methylpurine DNA glycosylase to induce alkylation damage cytotoxicity
    Article Snippet: The bacterial glycerol stocks harboring sequence-verified shRNA lentiviral plasmid vectors for human genes were selected from MISSION shRNA library (MilliporeSigma).

    Article Title: Systematic Evaluation Defines the Limits of Ferroptosis in Cancer Therapy
    Article Snippet: L-buthionine sulfoximine (B2515), L-buthionine sulfoxide (SC-207795, Santa Cruz), L-methionine sulfoximine (AC227202500, Fisher Scientific), L-methionine sulfoxide (36255-1, Cayman Chemical), Cystine (C7602, Sigma), Erastin (S7242, Selleckchem), RSL-3 (HY-100218A, MedChemExpress), beta-mercaptoethanol (NC9604560, Fisher Scientific), ferrostatin-1 (SML0583, Sigma), cycloheximide (14126, Cayman), eprenetapopt (gift from Aprea Therapeutics), DMSO (3176, Cell Signaling Technologies), sodium selenite (214485, Sigma), doxycycline (J60579.22, LifeSciences), ML-162 (HY-100002, MedChemExpress) ML-210 (HY-100003, MedChemExpress), DTT (D0632, Sigma), TCEP (C4706, Sigma), U-13C-serine (CLM-1574-H, Cambridge Isotopes), Rosiglitazone (71740, Cayman), (R)-HTS-3 (37563, Cayman), GSH-Glo (Promega V6911), Cell Titer Glo (Promega G7573), Cell Death Inducer Compound Library (35093, Cayman), Transwell culture apparatus (CLS3464, Sigma).

    Generated:

    Article Title: Loss of the kinases TBK1 and IKKε sensitizes target cancer cells to T cell killing through an IFN-γ–mediated inflammatory death program
    Article Snippet: .. Compound knockouts of B16 cells were generated by sequential lentiviral transduction with pLenticrispr v2 (a gift from Dr. Feng Zhang, Addgene #52961, puromycin selection), lenti-sgRNA blast (a gift from Dr. Brett Stringer, Addgene #104993, blasticidin selection), lenti-sgRNA neo (a gift from Dr. Brett Stringer, Addgene #104992, G418 selection), and lenti-sgRNA hygro (a gift from Dr. Brett Stringer, Addgene #104991, hygromycin selection). .. At each knockout and antibiotic selection stage, a corresponding non-targeting control guide was introduced using the same vector.

    Transduction:

    Article Title: Loss of the kinases TBK1 and IKKε sensitizes target cancer cells to T cell killing through an IFN-γ–mediated inflammatory death program
    Article Snippet: .. Compound knockouts of B16 cells were generated by sequential lentiviral transduction with pLenticrispr v2 (a gift from Dr. Feng Zhang, Addgene #52961, puromycin selection), lenti-sgRNA blast (a gift from Dr. Brett Stringer, Addgene #104993, blasticidin selection), lenti-sgRNA neo (a gift from Dr. Brett Stringer, Addgene #104992, G418 selection), and lenti-sgRNA hygro (a gift from Dr. Brett Stringer, Addgene #104991, hygromycin selection). .. At each knockout and antibiotic selection stage, a corresponding non-targeting control guide was introduced using the same vector.



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